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229e  (Sino Biological)


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    Structured Review

    Sino Biological 229e
    229e, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vg40605+cf/Human+coronavirus(HCoV-229E)+Spike+Gene+ORF+cDNA+clone+expression+plasmid(Codon+Optimized)%2C+C-Flag+tag/10__1172_slash_jci181136-288-32-33
    Average 93 stars, based on 9 article reviews
    229e - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: IgG targeting distinct seasonal coronavirus- conserved SARS-CoV-2 spike subdomains correlates with differential COVID-19 disease outcomes
    Article Snippet: Human coronavirus(HCoV-NL63) Spike Gene ORF cDNA clone expression plasmid(Codon Optimized), C-Flag tag , SinoBiological , VG40604-CF. .. Human coronavirus(HCoV-229E) Spike Gene ORF cDNA clone expression plasmid(Codon Optimized), C-Flag tag , SinoBiological , VG40605-CF. ..

    Plasmid Preparation:

    Article Title: IgG targeting distinct seasonal coronavirus- conserved SARS-CoV-2 spike subdomains correlates with differential COVID-19 disease outcomes
    Article Snippet: Human coronavirus(HCoV-NL63) Spike Gene ORF cDNA clone expression plasmid(Codon Optimized), C-Flag tag , SinoBiological , VG40604-CF. .. Human coronavirus(HCoV-229E) Spike Gene ORF cDNA clone expression plasmid(Codon Optimized), C-Flag tag , SinoBiological , VG40605-CF. ..

    Mutagenesis:

    Article Title: Rapid simulation of glycoprotein structures by grafting and steric exclusion of glycan conformer libraries.
    Article Snippet: Masiulis et al.22 PDB: 6HUO MERS-CoV S structure in complex with 5-N-acetyl neuraminic acid Park et al.90 PDB: 6Q04 Cryo-EM structure of MERS-CoV spike protein, all RBD-down conformation This paper PDB: 7YN0 Cryo-EM structure of MERS-CoV spike protein, intermediate conformation This paper PDB: 7YMZ Cryo-EM structure of MERS-CoV spike protein, One RBD-up conformation 1 This paper PDB: 7YMY Cryo-EM structure of MERS-CoV spike protein, One RBD-up conformation 2 This paper PDB: 7YMX Cryo-EM structure of MERS-CoV spike protein, all RBD-down conformation This paper EMD-33949 Cryo-EM structure of MERS-CoV spike protein, intermediate conformation This paper EMD-33948 Cryo-EM structure of MERS-CoV spike protein, One RBD-up conformation 1 This paper EMD-33947 Cryo-EM structure of MERS-CoV spike protein, One RBD-up conformation 2 This paper EMD-33946 Fourier-cropped cryo-EM map of SARS-CoV-2 S-D614G (corresponding to PDB:7EAZ and EMDB:EMD-31047) This paper EMD-38650 Experimental models: Cell lines Expi293F Cells ThermoFisher Cat# A14527 HEK293 Freestyle ThermoFisher Cat# R79007 Recombinant DNA pcDNA3.1 CD33-N-cadherin (EC1-EC5; residues 160-712) This paper Custom synthesis based on Uniprot ID: NP_031690.3 pcDNA3.1 CD33-N-cadherin (EC4-EC5; residues 331-542) This paper Custom synthesis based on Uniprot ID: NP_031690.3 SARS-CoV-2 S protein with the D614G mutation in pcDNA3.4-TOPO This paper Custom synthesis based on Uniprot ID: P0DTC2 SARS-CoV S protein with the 2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40150-G-N (GenBank ID: AAP13567.1) MERS-CoV S protein with the 2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40069-NF (GenBank ID: APF29071.1) (Continued on next page) ll OPEN ACCESS Cell 187, 1296–1311.e1–e15, February 29, 2024 e2 Resource .. REAGENT or RESOURCE SOURCE IDENTIFIER MERS-CoV S protein with the fm2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40069-NF (GenBank ID: AFS88936.1) hCoV-NL63 S protein with the 2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40604-CF (GenBank ID: APF29071.1) hCoV-229E S protein with the 2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40605-CF (GenBank ID: APT69883.1) hCoV-HKU1 S protein with the fm2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40606-UT (GenBank ID: Q0ZME7.1) Software and algorithms GlyCONFORMER Grothaus et al.38 https://github.com/IsabellGrothaus/ GlyCONFORMER Relion 3.1 Zivanov et al.91 https://www3.mrc-lmb.cam.ac.uk/ relion//index.php?title=Main_Page CryoSPARC v3.2 Punjani et al.92 https://cryosparc.com/ Phenix 1.19.2-4158 Adams et al.93 http://www.phenix-online.org/ Win Coot 0.9.4.1 Emsley et al.94 https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ UCSF-ChimeraX 1.2.5 Pettersen et al.95 https://www.rbvi.ucsf.edu/chimerax/ Byonic v3.9.6 Protein Metrics, USA https://proteinmetrics.com/byos/ Byos v3.11 Protein Metrics, USA https://proteinmetrics.com/byos/ ATSAS-3.0.3-1 Manalastas-Cantos et al.96 https://www.embl-hamburg.de/ biosaxs/software.html SAXS deduction program Shih et al.97 N/A Gromacs 2018.2 or newer Abraham et al.98 www.gromacs.org Gromaps Briones et al.99 https://mptg-cbp.github.io/ gromaps.html MDAnalysis-1.1.1 Gowers et al.100 https://www.mdanalysis.org/ numpy-1.21.1 Harris et al.101 Numpy.org Matplotlib-2.2.3 Hunter102 Matplotlib.org mrcfile-1.4.3 Burnley et al.103 https://mrcfile.readthedocs.io/en/ stable/index.html GlycoSHIELD program suite: GlycoSHIELD, GlycoTRAJ, GlycoSASA, GlycoALPHAFOLD This paper Zenodo: https://zenodo.org/records/ 10668375 GlycoDENSITY This paper www.glycoshield.eu Modeller-10.4 Webb and Sali104 https://salilab.org/modeller/ Other Superdex 200 Increase 10/300 GL Cytiva Cat# 28990944 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HisPur Cobalt Resin ThermoFisher Cat# 89966 Superose 6 Increase 10/300 GL Cytiva Cat# 29091596 Carbon film 300 mesh Cu Grids Electron Microscopy Sciences Cat# CF300-Cu-50 Quantifoil R1.2/1.3 300-mesh Cu Grids. .. Ted Pella Cat# 658-300-CU-100 ll OPEN ACCESS Resource

    Software:

    Article Title: Rapid simulation of glycoprotein structures by grafting and steric exclusion of glycan conformer libraries.
    Article Snippet: Masiulis et al.22 PDB: 6HUO MERS-CoV S structure in complex with 5-N-acetyl neuraminic acid Park et al.90 PDB: 6Q04 Cryo-EM structure of MERS-CoV spike protein, all RBD-down conformation This paper PDB: 7YN0 Cryo-EM structure of MERS-CoV spike protein, intermediate conformation This paper PDB: 7YMZ Cryo-EM structure of MERS-CoV spike protein, One RBD-up conformation 1 This paper PDB: 7YMY Cryo-EM structure of MERS-CoV spike protein, One RBD-up conformation 2 This paper PDB: 7YMX Cryo-EM structure of MERS-CoV spike protein, all RBD-down conformation This paper EMD-33949 Cryo-EM structure of MERS-CoV spike protein, intermediate conformation This paper EMD-33948 Cryo-EM structure of MERS-CoV spike protein, One RBD-up conformation 1 This paper EMD-33947 Cryo-EM structure of MERS-CoV spike protein, One RBD-up conformation 2 This paper EMD-33946 Fourier-cropped cryo-EM map of SARS-CoV-2 S-D614G (corresponding to PDB:7EAZ and EMDB:EMD-31047) This paper EMD-38650 Experimental models: Cell lines Expi293F Cells ThermoFisher Cat# A14527 HEK293 Freestyle ThermoFisher Cat# R79007 Recombinant DNA pcDNA3.1 CD33-N-cadherin (EC1-EC5; residues 160-712) This paper Custom synthesis based on Uniprot ID: NP_031690.3 pcDNA3.1 CD33-N-cadherin (EC4-EC5; residues 331-542) This paper Custom synthesis based on Uniprot ID: NP_031690.3 SARS-CoV-2 S protein with the D614G mutation in pcDNA3.4-TOPO This paper Custom synthesis based on Uniprot ID: P0DTC2 SARS-CoV S protein with the 2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40150-G-N (GenBank ID: AAP13567.1) MERS-CoV S protein with the 2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40069-NF (GenBank ID: APF29071.1) (Continued on next page) ll OPEN ACCESS Cell 187, 1296–1311.e1–e15, February 29, 2024 e2 Resource .. REAGENT or RESOURCE SOURCE IDENTIFIER MERS-CoV S protein with the fm2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40069-NF (GenBank ID: AFS88936.1) hCoV-NL63 S protein with the 2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40604-CF (GenBank ID: APF29071.1) hCoV-229E S protein with the 2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40605-CF (GenBank ID: APT69883.1) hCoV-HKU1 S protein with the fm2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40606-UT (GenBank ID: Q0ZME7.1) Software and algorithms GlyCONFORMER Grothaus et al.38 https://github.com/IsabellGrothaus/ GlyCONFORMER Relion 3.1 Zivanov et al.91 https://www3.mrc-lmb.cam.ac.uk/ relion//index.php?title=Main_Page CryoSPARC v3.2 Punjani et al.92 https://cryosparc.com/ Phenix 1.19.2-4158 Adams et al.93 http://www.phenix-online.org/ Win Coot 0.9.4.1 Emsley et al.94 https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ UCSF-ChimeraX 1.2.5 Pettersen et al.95 https://www.rbvi.ucsf.edu/chimerax/ Byonic v3.9.6 Protein Metrics, USA https://proteinmetrics.com/byos/ Byos v3.11 Protein Metrics, USA https://proteinmetrics.com/byos/ ATSAS-3.0.3-1 Manalastas-Cantos et al.96 https://www.embl-hamburg.de/ biosaxs/software.html SAXS deduction program Shih et al.97 N/A Gromacs 2018.2 or newer Abraham et al.98 www.gromacs.org Gromaps Briones et al.99 https://mptg-cbp.github.io/ gromaps.html MDAnalysis-1.1.1 Gowers et al.100 https://www.mdanalysis.org/ numpy-1.21.1 Harris et al.101 Numpy.org Matplotlib-2.2.3 Hunter102 Matplotlib.org mrcfile-1.4.3 Burnley et al.103 https://mrcfile.readthedocs.io/en/ stable/index.html GlycoSHIELD program suite: GlycoSHIELD, GlycoTRAJ, GlycoSASA, GlycoALPHAFOLD This paper Zenodo: https://zenodo.org/records/ 10668375 GlycoDENSITY This paper www.glycoshield.eu Modeller-10.4 Webb and Sali104 https://salilab.org/modeller/ Other Superdex 200 Increase 10/300 GL Cytiva Cat# 28990944 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HisPur Cobalt Resin ThermoFisher Cat# 89966 Superose 6 Increase 10/300 GL Cytiva Cat# 29091596 Carbon film 300 mesh Cu Grids Electron Microscopy Sciences Cat# CF300-Cu-50 Quantifoil R1.2/1.3 300-mesh Cu Grids. .. Ted Pella Cat# 658-300-CU-100 ll OPEN ACCESS Resource

    Electron Microscopy:

    Article Title: Rapid simulation of glycoprotein structures by grafting and steric exclusion of glycan conformer libraries.
    Article Snippet: Masiulis et al.22 PDB: 6HUO MERS-CoV S structure in complex with 5-N-acetyl neuraminic acid Park et al.90 PDB: 6Q04 Cryo-EM structure of MERS-CoV spike protein, all RBD-down conformation This paper PDB: 7YN0 Cryo-EM structure of MERS-CoV spike protein, intermediate conformation This paper PDB: 7YMZ Cryo-EM structure of MERS-CoV spike protein, One RBD-up conformation 1 This paper PDB: 7YMY Cryo-EM structure of MERS-CoV spike protein, One RBD-up conformation 2 This paper PDB: 7YMX Cryo-EM structure of MERS-CoV spike protein, all RBD-down conformation This paper EMD-33949 Cryo-EM structure of MERS-CoV spike protein, intermediate conformation This paper EMD-33948 Cryo-EM structure of MERS-CoV spike protein, One RBD-up conformation 1 This paper EMD-33947 Cryo-EM structure of MERS-CoV spike protein, One RBD-up conformation 2 This paper EMD-33946 Fourier-cropped cryo-EM map of SARS-CoV-2 S-D614G (corresponding to PDB:7EAZ and EMDB:EMD-31047) This paper EMD-38650 Experimental models: Cell lines Expi293F Cells ThermoFisher Cat# A14527 HEK293 Freestyle ThermoFisher Cat# R79007 Recombinant DNA pcDNA3.1 CD33-N-cadherin (EC1-EC5; residues 160-712) This paper Custom synthesis based on Uniprot ID: NP_031690.3 pcDNA3.1 CD33-N-cadherin (EC4-EC5; residues 331-542) This paper Custom synthesis based on Uniprot ID: NP_031690.3 SARS-CoV-2 S protein with the D614G mutation in pcDNA3.4-TOPO This paper Custom synthesis based on Uniprot ID: P0DTC2 SARS-CoV S protein with the 2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40150-G-N (GenBank ID: AAP13567.1) MERS-CoV S protein with the 2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40069-NF (GenBank ID: APF29071.1) (Continued on next page) ll OPEN ACCESS Cell 187, 1296–1311.e1–e15, February 29, 2024 e2 Resource .. REAGENT or RESOURCE SOURCE IDENTIFIER MERS-CoV S protein with the fm2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40069-NF (GenBank ID: AFS88936.1) hCoV-NL63 S protein with the 2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40604-CF (GenBank ID: APF29071.1) hCoV-229E S protein with the 2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40605-CF (GenBank ID: APT69883.1) hCoV-HKU1 S protein with the fm2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40606-UT (GenBank ID: Q0ZME7.1) Software and algorithms GlyCONFORMER Grothaus et al.38 https://github.com/IsabellGrothaus/ GlyCONFORMER Relion 3.1 Zivanov et al.91 https://www3.mrc-lmb.cam.ac.uk/ relion//index.php?title=Main_Page CryoSPARC v3.2 Punjani et al.92 https://cryosparc.com/ Phenix 1.19.2-4158 Adams et al.93 http://www.phenix-online.org/ Win Coot 0.9.4.1 Emsley et al.94 https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ UCSF-ChimeraX 1.2.5 Pettersen et al.95 https://www.rbvi.ucsf.edu/chimerax/ Byonic v3.9.6 Protein Metrics, USA https://proteinmetrics.com/byos/ Byos v3.11 Protein Metrics, USA https://proteinmetrics.com/byos/ ATSAS-3.0.3-1 Manalastas-Cantos et al.96 https://www.embl-hamburg.de/ biosaxs/software.html SAXS deduction program Shih et al.97 N/A Gromacs 2018.2 or newer Abraham et al.98 www.gromacs.org Gromaps Briones et al.99 https://mptg-cbp.github.io/ gromaps.html MDAnalysis-1.1.1 Gowers et al.100 https://www.mdanalysis.org/ numpy-1.21.1 Harris et al.101 Numpy.org Matplotlib-2.2.3 Hunter102 Matplotlib.org mrcfile-1.4.3 Burnley et al.103 https://mrcfile.readthedocs.io/en/ stable/index.html GlycoSHIELD program suite: GlycoSHIELD, GlycoTRAJ, GlycoSASA, GlycoALPHAFOLD This paper Zenodo: https://zenodo.org/records/ 10668375 GlycoDENSITY This paper www.glycoshield.eu Modeller-10.4 Webb and Sali104 https://salilab.org/modeller/ Other Superdex 200 Increase 10/300 GL Cytiva Cat# 28990944 HiLoad 16/600 Superdex 75 pg Cytiva Cat# 28989333 HisPur Cobalt Resin ThermoFisher Cat# 89966 Superose 6 Increase 10/300 GL Cytiva Cat# 29091596 Carbon film 300 mesh Cu Grids Electron Microscopy Sciences Cat# CF300-Cu-50 Quantifoil R1.2/1.3 300-mesh Cu Grids. .. Ted Pella Cat# 658-300-CU-100 ll OPEN ACCESS Resource



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    Image Search Results


    Journal: eLife

    Article Title: Murine alveolar macrophages rapidly accumulate intranasally administered SARS-CoV-2 Spike protein leading to neutrophil recruitment and damage

    doi: 10.7554/eLife.86764

    Figure Lengend Snippet:

    Article Snippet: Recombinant DNA reagent , Human coronavirus (HCoV-229E) Spike Gene ORF cDNA clone expression plasmid (Codon Optimized) HCoV-229E , SinoBiological , VG40605-UT , .

    Techniques: Blocking Assay, Imaging, Staining, Recombinant, Plasmid Preparation, Expressing, Luciferase, Cell Culture, Transfection

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Differences in syncytia formation by SARS-CoV-2 variants modify host chromatin accessibility and cellular senescence via TP53

    doi: 10.1016/j.celrep.2023.113478

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: 229E-CoV (VG40605-CF), HKU1-CoV (VG40021-UT), NL63-CoV (VG40604-CF), OC43-CoV (VG40607-CF), B.1.1.7 SARS-CoV-2 (VG40771-CF), and B.1.617.2 SARS-CoV-2 (VG40804-UT) spike plasmids were obtained from Sino Biological and subcloned into pcDNA3.1-puro.

    Techniques: FLAG-tag, Luciferase, Virus, Recombinant, SYBR Green Assay, Activity Assay, Software, CRISPR